利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素(somatostatin,SST)基因(sst1)突变体鱼系。通过比较6 dpf(days post fertilization,dpf)的sst1^(-/-)突变体和野生型仔鱼转录组,发现sst1^(-/-)突变体相关生化过程和信号通路发生了显著变化。相比野生型,sst1^(-/-)突变体仔鱼中有354个基因显著上调,504个基因显著下调。GO(gene ontology)富集分析表明,大部分差异基因与氨基酸和蛋白质生物合成相关。KEGG(kyoto encyclopedia of genes and genomes)通路富集显示代谢通路、氨基酸合成、氨基酰基-tRNA合成、核糖体合成和rRNA加工相关通路表达增强。结果表明,sst1^(-/-)突变体蛋白质生物合成和代谢过程更加活跃。RT-qPCR验证结果显示,随机选取的10个基因mRNA表达变化趋势与转录组测序结果一致,说明转录组测序结果真实可靠。6月龄突变体体质量、体长和野生型相比并无显著差异。本研究结果为进一步挖掘生长抑素基因家族的潜在功能奠定基础。
[Objective] The paper aims to study the attractive effects of 12 Chinese medicinal herbs on Scophthalmus maximus juvenile.[Method] We took 200 Scophthalmus maximus with the average body length of 3 cm,average weight of 1 g and 60 days of age as the research object,and 12 Chinese medicinal herbs as feed attractants were selected,including Cloves,Cinnamon,Star anise,Kaempferol,Mint,Coptis,Phellodendron,skullcap,Gardenia,Sophora flavescens,Mume,Myrobalan meat were selected.The amount added to the basic diet was 2‰..The times of turbot juveniles going into each inducing room and the times of turbot juveniles biting the feed ball were recorded by maze experiments.Inducing effects of the Chinese herbal medicines were analyzed.[Result] The results showed that the attractant effect of Mume,Gardenia,Scutellaria and kaempferol had the significantly inducing effect on Scophthalmus maximus juvenile.And 2‰ Gardenia,5‰ Matrine,5‰ Scutellaria and 2‰ Kaempferol have the best attractant effects.In compound experients,the attractive effect of mixture of 2‰ matrine and gardenia on Scophthalmus maximus was the best.[Conclusion] The attractive effect on Scophthalmus maximus has something to do with the kind and concentration of Chinese medicinal herbs.
