In this study,a 1.35 kb DNA fragment encoding the VP2 protein of infectious bursal disease CJ801 isolate was obtained by PCR and the sequence was determined in T vector.The VP2 gene was cloned into the vector of pcDNATM4/His/LacZ,then the expression cassette including the VP2 gene of IBDV and LacZ gene of E.coli controlled by CMV promoter were inserted into US2 gene of MDV CVI988/Rispens to give a transfer vector of pUS2-VP2.The complex of pUS2-VP2 and DOTAP was transfected into MDV infected CEF.The recombinant MDV expressing LacZ gene were selected and purified on 96-well plate by blue plague.The complete VP2 gene inserted into MDV was detected by PCR.Western blot and immunostaining results demonstrated that VP2 protein of IBDV was expressed by recombinant MDV.
