基于液相芯片(Multi-analyte suspension array,MASA)技术建立一种同时对H5N1和H7N9亚型流感病毒进行快速检测的新方法。对国家生物技术信息中心(National center for biotechnology information,NCBI)和欧洲生物信息学中心(The european bioinformatics institute,EBI)核酸数据库中已有的H5、N1、H7和N9核酸片段进行比对分析,并参考世界卫生组织,美国疾病预防控制中心和中国疾病预防控制中心等的相关资料,设计简并引物和探针,将合成的探针与荧光编码微球进行偶联,建立检测方法。利用该方法检测H5N1和H7N9亚型流感病毒和其它常见亚型(H1N1、甲流H1N1、H5N2、BH3N2和H9N2)的标本。建立了一种快速的流感病毒分型方法。这种方法可以同时检测H5N1和H7N9,用已知基因型的样本对这种方法进行验证显示它的特异性很好。灵敏度分析实验也表明这种方法具有很高的灵敏度,可以对含有5个拷贝的样本进行有效的检测。利用液相芯片技术研制的H5N1和H7N9亚型流感病毒检测方法能够用于流感病毒H5N1和H7N9亚型的快速、灵敏、特异性的检测及鉴定。
INTRODUCTION Herpes simplex virus (HSV) is a ubiquitous human pathogen that causes a range of diseases from mild uncomplicated mucocutaneous infection to life-threatening conditions.1 HSV-1 is normally associated with orofacial infections,whereas HSV-2 usually causes genital infections and can be transmitted from infected mothers to neonates.2 Less common manifestations of HSV infection,such as meningitis,encephalitis,3,4 hepatitis,5 and pneumonitis,6,7 can occur in both children and adults.After primary infection by HSV,the virus establishes a lifelong latent infection in the neuronal district,resulting in reactivation due to various triggering factors.
